SCANDNAnet- Validating metabarcoding for routine use in Nordic freshwater biomonitoring

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Projektbeskrivning:

In the last years, the advancements in High Throughput Sequencing (HTS) led to a major breakthrough in DNA-based species identification of bulk samples. The new technique, DNA metabarcoding, identifies hundreds of specimens present in environmental bulk samples on species level. Metabarcoding differs from traditional barcoding in the ability to use entire communities or environmental samples, the sequencing technologies involved, and the data processing [8,9]. In DNA metabarcoding for aquatic macroinvertebrates, the complete sample is homogenized and DNA extracted in bulk, followed by target marker amplification and high-throughput sequencing. From dozens of samples, millions of barcode sequences are generated in a single run, bioinformatically processed and sequences clustered into ‘operational taxonomic units’ (OTUs). The DNA metabarcoding method provides accurate, standardized and high-resolution taxonomic data [8,10,11]. Using reference databases such as BOLD (www.boldsystems.org)

Senast uppdaterad:
2022-10-25 16:29